Fluorometer FL 6000
Customized your Screening for Photosynthetic Performance
About...

Double-Modulation Fluorometer FL 6000 measures chlorophyll α fluorescence. Measured fluorescence emission is excited by a set of light-emitting diodes that generate measuring flashes of few microseconds in duration and low light intensity; the photochemistry is driven by single-turnover flashes or by continuous actinic irradiance. Chlorophyll α fluorescence is detected by a PIN photodiode and digitized by a 16-bit A/D converter.

Key Features

  • SuperHead measuring unit –measurements performed in standard cuvette for sample suspensions of algae or cyanobacteria
  • Unifed control unit for all FL 6000 versions, compatible with different measuring units.
  • Light intensities and timings are software controlled separately for each LED set with 100 ns resolution


  • Four sets of LEDs:
  • - measuring light 1 – red
    - measuring light 2 – blue
    - actinic light – red
    - single-turnover flash – red

  • PIN photodiode detector with 40× variable gain for signal acquisition with 1MHz/16-bit maximal accuracy
  • Pulse Amplitude Modulation measurements

Fluorometer FL 6000


Double-Modulation Fluorometer FL 6000 measures chlorophyll α (Chlα)- -fluorescence. Measured fluorescence emission is excited by a set of light- -emitting diodes that generate measuring flashes of few microseconds in duration and low intensity; the photochemistry is driven by single-turnover flashes or by continuous actinic irradiance. Chlorophyll fluorescence is detected by a PIN photodiode and digitized by a 16-bit A/D converter. The instrument supports Pulse Amplitude Modulation measurements and, at the same time, can capture fast OJIP transients or perform rapid measurements of QA- reoxidation kinetics, S-states.

The fast version of FL 6000-F allows measuring Flash Fluorescence Induction (FFI) experiments which leads to a calculation of the effective antenna size, antenna connectivity and rapid lights curves. The major feature of this fluorometer is the capacity of the instrument to generate rectangular actinic flashes of extremely high power also with 620 nm excitation light source. This feature is helpful for cyanobacteria measurements. Full reduction of QA acceptor can be achieved within 25 µs and the instrument can measure fluorescence induction during such a single-turnover saturating flash. This technique is used to determine the effective antenna size of the Photosystem II as well as its heterogeneity and connectivity without disturbing the measured system by DCMU or other herbicides.


Photosynthetically active samples, either suspensions, leaves, or even corals, can be investigated for their photochemical yields, quenching parameters, state transitions, or for the kinetics of photosynthetic redox reactions. The FL 6000 has two input channels. First channel is used for Chlα-fluorescence measurements. The second channel can be optionally used for temperature reading or dissolved oxygen probe signal measurements. FAR-LED unit with 735 nm LEDs can be optionally connected to the SuperHead measuring unit. Thermoregulator TR 2000 or dissolved O2 microelectrode A/D converter are optional accessories for measurements with SuperHead measuring unit.


Applications


  • Probing physiology of photoautotrophs suspensions
  • Measurement of efficiency of PSII photochemistry
  • Estimation of aquatic primary productivity
  • Exploring photosynthetic performance and metabolic perturbations in phytoplankton
  • Molecular biology – screening for photosynthetic mutants • Detection of abiotic and biotic stress and stress tolerance
  • Taxonomical studies
  • Aquatic bloom detection



Software


  • Creation and archivation of experimental protocols
  • FluorWin Wizard for automated protocols
  • Retrieval and export of experimental data
  • Data manipulation and visualization
  • Windows 7, or higher compatible



Versions


FLUOROMETER FL 6000-S


  • Time resolution up to 4 µs
  • Detection limit 100 ng Chlα/l
  • Supported fluorescence protocols:
  • - Instantaneous fluorescence
    - QA- reoxidation kinetics
    - Kautsky effect
    - Quenching parameters analysis
    - Fast OJIP transient
    - S-states



FLUOROMETER FL 6000-F


  • Time resolution up to 1 µs
  • Detection limit 1 mg Chlα/l
  • Supported all standard protocols
  • Special fluorescence protocol:
  • - Flash Fluorescence Induction (FFI)


Specifications


Measured fluorescence parameters:
F0, FM, FV, F’0, F’M, F’V, FT

Light Sources:
620 nm and 460 nm in standard versions; other wavelengths available•

Single Turnover Flash:
100,000 µmol.m-2.s-1 (max. duration 150 µs)

Actinic Light Irradiance:
Up to 3,000 µmol.m-2.s-1

Custom-Defned Protocols:
Variable timing, special language and scripts

A/D bit Resolution:
16 bit

Detector time response:
1 µs (FL 6000-F), 4 µs (FL 6000-S)

Communication Port:
RS 232 with USB port converter

Control box FL 6000 dimension:
29 × 20 × 11 cm

Measuring unit dimension:
SuperHead: diameter 16 × 6 cm;

Total weight:
Appr. 5 kg

Power Input:
20 W

Electrical:
90 – 240 V

Download

Documents


Software


Optional features and accessories


Termoregulator TR 2000


  • precise temperature control
  • range of 0 °C to +70 °C
  • accuracy of 0.1 °C
  • mode of regulation constant or temperature ramp
Provides precise temperature control in the range of 0 °C to +70 °C with an accuracy of 0.1 °C. The actual temperature is displayed on the front panel of the device. It includes control unit and temperature controller. TR 2000 can work in two modes: (i) constant mode, (ii) temperature ramp mode. In the constant mode, the instrument maintains a constant temperature of the measured sample. The temperature ramp mode enables linear changing of the sample temperature with a rate ranging from 0.1 °C/sec to 1 °C/sec.


Magnetic stirrer


  • constant temperature or prevent sedimentation
Magnetic Stirrer is designed to provide continuous stirring with little speed deviation and minimum heat build-up. Continuous, uniform stirring is essential for keeping a constant temperature within the entire sample volume when the temperature control is applied. The rate of stirring is set by a knob on the front panel of the device. Magnetic Stirrer is a handy accessory to PSI Fluorometers: it can be connected to the Fluorometer Control Unit and controlled by it (switched on and off). Two stirring bars are included in the price.


Oxygen detector with oxygen electrode


  • online oxygen evolution measurement
Serves for oxygen evolution detection based on dynamic quenching of fluorescence (electrode sold separately). Important notice: Mechanical construction of the fluorometer does not allow simultaneous measurement of fluorescence and oxygen evolution.
NOTE: It is highly recommended to use the oxygen detector module with the Magnetic Stirrer.

Oxygen Electrode *
Supplement to oxygen detector module.



Infra-Red LED Unit


  • additional external light source
  • peak wavelength 730 nm
  • for correct F’0 determination
Allows measuring Fo' and PAR absorbance. It is also used during quenching analysis protocol.


PC with Preinstalled Control Software *


Notebook PC with preinstalled software for fluorometer control.


Additional SuperHead


Fluorometer standard configuration can be enhanced by adding a second SuperHead. This SuperHead can be constructed with respect to customer's experimental needs: specific LED colors, which cannot be added to the first SuperHead (Blue, Cyan, Amber) and detection bands (ChlA, ChlB).

Pricing


Fluorometer
Version Characteristics Price
Fast Fluorometer FL 6000-F Includes measuring optical head with a cuvette, multipurpose control unit, power supply, FluorWin software, user’s guide. 9,990 €
Standard Fluorometer FL 6000-S Includes measuring optical head with a cuvette, multipurpose control unit, power supply, FluorWin software, user’s guide. 8,490 €

Optional Accessories

Additional SuperHead Fluorometer standard configuration can be enhanced by adding a second SuperHead. This SuperHead can be constructed with respect to customer's experimental needs: specific LED colors (Blue, Cyan, Amber) and detection bands (ChlA, ChlB). 3,490 €
Infra-Red LED Unit Allows measuring Fo' and PAR absorbance. It is also used during quenching analysis protocol. 1,490 €
Magnetic Stirrer For uniform sample stirring. Can be controlled manually or by the Fluorometer Control Unit. Two stirring bars are included in the price. 998 €
Oxygen Detector Module Serves for oxygen evolution detection based on dynamic quenching of fluorescence (no electrode included). 1,550 €
Oxygen Electrode * A supplement to oxygen detector module. 690 €
PC with Preinstalled Control Software * Notebook PC with preinstalled software. 390 €
Thermoregulator TR 2000 Includes control unit and temperature controller. 2,999 €