Double-Modulation Fluorometer FL 6000 measures chlorophyll α ﬂuorescence. Measured ﬂuorescence emission is excited by a set of light-emitting diodes that generate measuring ﬂashes of few microseconds in duration and low light intensity; the photochemistry is driven by single-turnover ﬂashes or by continuous actinic irradiance. Chlorophyll α ﬂuorescence is detected by a PIN photodiode and digitized by a 16-bit A/D converter.
The fast version of FL 6000-F allows measuring Flash Fluorescence Induction (FFI) experiments which leads to a calculation of the effective antenna size, antenna connectivity and rapid lights curves. The major feature of this ﬂuorometer is the capacity of the instrument to generate rectangular actinic ﬂashes of extremely high power also with 620 nm excitation light source. This feature is helpful for cyanobacteria measurements. Full reduction of QA acceptor can be achieved within 25 µs and the instrument can measure ﬂuorescence induction during such a single-turnover saturating ﬂash. This technique is used to determine the effective antenna size of the Photosystem II as well as its heterogeneity and connectivity without disturbing the measured system by DCMU or other herbicides.
Photosynthetically active samples, either suspensions, leaves, or even corals, can be investigated for their photochemical yields, quenching parameters, state transitions, or for the kinetics of photosynthetic redox reactions. The FL 6000 has two input channels. First channel is used for Chlα-ﬂuorescence measurements. The second channel can be optionally used for temperature reading or dissolved oxygen probe signal measurements. FAR-LED unit with 735 nm LEDs can be optionally connected to the SuperHead measuring unit. Thermoregulator TR 2000 or dissolved O2 microelectrode A/D converter are optional accessories for measurements with SuperHead measuring unit.
620 nm and 460 nm in standard versions; other wavelengths available•
Single Turnover Flash:
100,000 µmol.m-2.s-1 (max. duration 150 µs)
Actinic Light Irradiance:
Up to 3,000 µmol.m-2.s-1
Variable timing, special language and scripts
A/D bit Resolution:
Detector time response:
1 µs (FL 6000-F), 4 µs (FL 6000-S)
RS 232 with USB port converter
Control box FL 6000 dimension:
29 × 20 × 11 cm
Measuring unit dimension:
SuperHead: diameter 16 × 6 cm;
Appr. 5 kg
90 – 240 V
Oxygen Electrode *
Supplement to oxygen detector module.
|Fast Fluorometer FL 6000-F||Includes measuring optical head with a cuvette, multipurpose control unit, power supply, FluorWin software, user’s guide.||9,990 €|
|Standard Fluorometer FL 6000-S||Includes measuring optical head with a cuvette, multipurpose control unit, power supply, FluorWin software, user’s guide.||8,490 €|
|Additional SuperHead||Fluorometer standard configuration can be enhanced by adding a second SuperHead. This SuperHead can be constructed with respect to customer's experimental needs: specific LED colors (Blue, Cyan, Amber) and detection bands (ChlA, ChlB).||3,490 €|
|Infra-Red LED Unit||Allows measuring Fo' and PAR absorbance. It is also used during quenching analysis protocol.||1,490 €|
|Magnetic Stirrer||For uniform sample stirring. Can be controlled manually or by the Fluorometer Control Unit. Two stirring bars are included in the price.||998 €|
|Oxygen Detector Module||Serves for oxygen evolution detection based on dynamic quenching of fluorescence (no electrode included).||1,550 €|
|Oxygen Electrode *||A supplement to oxygen detector module.||690 €|
|PC with Preinstalled Control Software *||Notebook PC with preinstalled software.||390 €|
|Thermoregulator TR 6000||Precise temperature control of liquid sample. Temperature range
from 5 °C to +60 °C.